{"id":47,"date":"2015-10-16T03:34:08","date_gmt":"2015-10-16T03:34:08","guid":{"rendered":"http:\/\/ventricular.org\/StephenNoctor\/?page_id=47"},"modified":"2018-12-07T22:08:49","modified_gmt":"2018-12-07T22:08:49","slug":"time-lapse-movies","status":"publish","type":"page","link":"https:\/\/ventricular.org\/StephenNoctor\/time-lapse-movies\/","title":{"rendered":"Time-Lapse Movies"},"content":{"rendered":"<div class=\"desktop-video\">\n<h2>2004 Nature Neuroscience Publication<\/h2>\n<p>[masterslider id=&#8221;8&#8243;]<\/p>\n<hr \/>\n<h2 style=\"text-align: left;\">2008 Journal of Comparative Neurology Publication<\/h2>\n<p>[masterslider id=&#8221;10&#8243;]<\/p>\n<hr \/>\n<h2 style=\"text-align: left;\">\u00a0More Videos<\/h2>\n<p>[masterslider id=&#8221;11&#8243;]<\/p>\n<hr \/>\n<\/div>\n<div class=\"mobile-video\">\n<h1>2004 Nature Neuroscience Publication<\/h1>\n<h4 style=\"text-align: center;\">Translocation of a radial glial cell out of the ventricular zone.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-1\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/tRG-the-first-Again.mp4?_=1\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/tRG-the-first-Again.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/tRG-the-first-Again.mp4<\/a><\/video><\/div>\n<h6>Among other projects I worked on as a graduate student in Sharon Juliano&#8217;s lab, I studied radial glial (RG) cell morphology and translocating RG cell morphology in the developing ferret brain. My education was informed by observation and literature, including Pasko Rakic&#8217;s 1979 &#8220;A Golgi Study of Radial Glial Cells in Developing Monkey Telencephalon: Morphogenesis and Transformation into Astrocytes&#8221;, and Thomas Voigt&#8217;s 1989 &#8220;Development of Glial Cells in the Cerebral Wall of Ferrets: Direct Tracing of Their Transformation From Radial Glia Into Astrocytes&#8221;. One question we examined in the Juliano lab was whether it was possible to alter RG cell morphology: could we induce translocating RG cells to revert back to the bipolar morphology of RG cells in the ventricular zone, and if so would that alter RG cell function? Those experiments continue in Sharon&#8217;s lab. I carried my enthusiasm for cortical development and radial glial cells along with me to Arnold Kriegstein&#8217;s lab where I performed my post-doctoral studies. I had been recording time-lapse videos of radial glial cells in vitro for over a year in Arnold&#8217;s lab when I recorded this time-lapse movie of a translocating radial glial cell in November, 2002.<\/h6>\n<h6>I published a protocol describing the time-lapse method I developed to record RG cell behaviors and phenotype daughter cells. Click here to read the protocol &#8211; it includes step by step instructions and a few photos of my setup. Briefly, I labeled embryonic rat RG cells through in utero retroviral injection on E15 or E16, prepared organotypic slices one day later and began recording immediately. The recordings lasted several days &#8211; in this experiment 5 days. This time-lapse sequence was included in my 2004 Nature Neuroscience publication. The movie captures the final RG cell division at the surface of the ventricle and subsequent translocation away from the ventricle toward the cortical plate. The RG cell appears to continue dividing away from the ventricle since clonal number increases. In my embryonic time-lapse recordings (all performed in embryonic rat neocortex), the RG cells that translocated away from the ventricle would only do so after first undergoing a final division at the VZ surface.<\/h6>\n<h6>At the time I made this recording there were reports that translocating cells in the developing brain were neurons, rather than a form of radial glial cell. To test this idea, after time-lapse recording I fixed the slice and immunostained with anti-TuJ1 antibodies. However the antibodies did not penetrate far enough into the organotypic slice to reach the clonal cells and I was not able to answer that question in this experiment. I therefore immunostained many fixed sections of embryonic rat neocortex that had developed in vivo, rather than in culture. None of the translocating RG cells in vivo expressed neuronal markers. Instead many expressed detectable levels of GFAP in the soma. In subsequent time-lapse experiments I used electrophysiological recordings, rather than immunostaining, to phenotype translocating cells. The E-phys recordings showed that translocating RG cells were not neurons &#8211; they had the membrane properties of astroglial cells. The E-phys recordings of translocating RG cells were included in the 2004 Nat. Neuroscience article. These data were consistent with earlier publications showing RG cell translocation and transformation into astroglial cells by Rakic, Voigt and others.<\/h6>\n<h4 style=\"text-align: center;\">Cortical neurons exhibit four stages of migration.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-2\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-neuronal-migration.mp4?_=2\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-neuronal-migration.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-neuronal-migration.mp4<\/a><\/video><\/div>\n<h6>This time-lapse video shows a cortical neuron, as a single cell clone, migrating from the proliferative zones to the cortical plate over 4 days of recording. The time-lapse approach revealed that rather than migrating directly from the ventricle to the cortical plate, newborn cortical neurons in the dorsal telencephalon went through stages of migration that involved a multipolar phase, and a retrograde movement toward the ventricle before commencing radial migration toward the cortical plate.<\/h6>\n<h4 style=\"text-align: center;\">Intermediate progenitor cells in the SVZ produce pairs of cortical neurons.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-3\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-IP-Neuron-pairs.mp4?_=3\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-IP-Neuron-pairs.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-IP-Neuron-pairs.mp4<\/a><\/video><\/div>\n<h6>These three videos show symmetric pairs of cortical neurons migrating toward the cortical plate. The sister neurons were produced by the terminal symmetric divisions of intermediate progenitor cells. These cortical neurons also demonstrated retrograde movements toward the ventricle before commencing radial migration toward the cortical plate. Ventricular-directed behavior had previously been reported for cortical interneurons migrating to the dorsal cortex by the Parnavelas lab. The retrograde movements toward the ventricle therefore appear to be a common behavior among migrating cortical neurons.<\/h6>\n<h4 style=\"text-align: center;\">Migrating neurons grow lengthy axonal processes before reaching the cortical plate.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-4\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-axonal-outgrowth.mp4?_=4\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-axonal-outgrowth.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/record-axonal-outgrowth.mp4<\/a><\/video><\/div>\n<h6>This time-lapse movie shows the outgrowth of an axonal process for a cortical neuron during the multipolar phase of migration.<\/h6>\n<h1>2008\u00a0Journal of Comparative Neurology Publication<\/h1>\n<h4 style=\"text-align: center;\">Vertical divisions produce asymmetric daughter cells.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-5\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/RG-IP-Neurons1.mp4?_=5\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/RG-IP-Neurons1.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/RG-IP-Neurons1.mp4<\/a><\/video><\/div>\n<h6 style=\"text-align: justify;\"><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/Cleavage-plan-AngleB.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-574 size-full\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/Cleavage-plan-AngleB.jpg\" alt=\"Cleavage plan AngleB\" width=\"200\" height=\"123\" \/><\/a>The goal of this project was to test the relationship between the angle\u00a0of dividing precursor cells and daughter cell fate. Background: If you examine mitoses at the surface of the lateral ventricle, you&#8217;ll notice\u00a0that most divide with a vertical orientation, but\u00a0that some\u00a0divide with oblique or horizontal orientations (see figure). It is\u00a0thought that the different orientations represent different types of divisions. The model, as it relates to the mammalian neocortex, predicts that a vertical cleavage plane orientation is associated with symmetric divisions, while a horizontal orientation is associated with asymmetric, neurogenic divisions. This mechanism\u00a0is thought to\u00a0segregate fate determinants and thus determine daughter cell fate.<\/h6>\n<h6><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/frederick_sauer_02B.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-thumbnail wp-image-573\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/frederick_sauer_02B-150x150.jpg\" alt=\"frederick_sauer_02B\" width=\"150\" height=\"150\" \/><\/a>This concept has been around for a very long time. Many have\u00a0addressed\u00a0the topic. In his\u00a01935 paper &#8220;<span style=\"color: #808080;\"><em><a style=\"color: #808080;\" href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/Sauer-1935-Mitosis-in-the-Neural-Tube.pdf\">Mitosis in the Neural Tube<\/a><\/em><\/span>&#8221; Frederick Sauer (pictured at left) wrote\u00a0that the number of horizontal divisions at the ventricle was not sufficient to account for the number of cortical neurons. Yet, the hypothesis remained attractive and was\u00a0supported by work on ganglion mother cells in Drosophila showing\u00a0that cleavage plane of dividing precursor cells is correlated with daughter cell fate.<\/h6>\n<h6>But, the idea had not been tested in mammalian neocortex in experiments that recorded\u00a0cleavage plane angle during division and then tracked\u00a0daughter cells over sufficient time for daughter cells to mature and reliably express phenotype. So, I labeled precursor cells with eGFP and prepared organotypic slices at early (E13-E15) or\u00a0late stages (E16-E19) of rat cortical development. I recorded angle of division in the VZ and SVZ. After several days of time-lapse recording I phenotyped\u00a0daughter cells through a combination of morphology, behavior, and Ephys\u00a0recordings.<\/h6>\n<h6>The results showed major differences between mode of division in the VZ and SVZ.\u00a0This movie shows a\u00a0vertical RG cell division at the ventricle &#8211; at the t=2h 51m mark &#8211; producing asymmetric daughter cells. One daughter is a self renewed RG cell that inherits the pial fiber and returns to the ventricle to divide again. The second daughter is an\u00a0intermediate progenitor cell that migrates away from the ventricle and divides horizontally in the SVZ to produce 2 cortical\u00a0neurons.<\/h6>\n<h6>These experiments\u00a0demonstrated that vertical divisions can produce asymmetric daughter cells, and that vertical divisions were not perfectly vertical &#8211; they averaged 82.9\u00ba with the cleavage plane always tilting away from the daughter cell that inherited\u00a0the RG pial fiber.\u00a0The results were based on 36 RG cell divisions and 24 IP cell divisions recorded via\u00a0time-lapse in vitro, and in vivo cleavage plane angle measurements of 1536 ventricular surface divisions and 879 SVZ divisions.<\/h6>\n<h6>In sum,\u00a0angle of division did not predict daughter cell fate. Instead, daughter cell fate was correlated\u00a0with\u00a0the stage of neurogenesis and the location of division: Divisions at the ventricle during early stages of cortical development were mostly symmetric, while during later stages divisions at the ventricle were mostly asymmetric, regardless of orientation. After the onset of neurogenesis, divisions that occurred at the ventricle \u00a0were largely asymmetric and those in the SVZ were largely symmetric. This suggests that inheritance of fate determinants is not controlled by the angle of division, but by other factors I&#8217;d be happy to discuss if you give me a call.<\/h6>\n<h4 style=\"text-align: center;\">Vertical divisions produce translocating radial glial cells that remain proliferative.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-6\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-trg1-real.mp4?_=6\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-trg1-real.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-trg1-real.mp4<\/a><\/video><\/div>\n<h6 style=\"text-align: justify;\">Vertically produced, asymmetric translocating radial glial cell. \u00a0This time-lapse movie is another example showing that vertical divisions can produce asymmetric daughter cells. \u00a0The RG cell divided vertically at the t=24h:30m mark, yet produced morphologically asymmetric daughter cells: one daughter cell retained the pial fiber and the second daughter cell remained close to the mother RG cell over 100+ hours of time-lapse recording. \u00a0After division the RG cell detached from the ventricle and translocated toward the cortical plate. \u00a0The translocating RG cell remained proliferative, dividing two additional times at the t=52h time point, and the t=83h time point. \u00a0After 100+ hours of time-lapse recording I transferred the organotypic slice to an Ephys rig and obtained whole-cell patch-clamp recordings from each cell. \u00a0All four cells exhibited the membrane properties of astroglial cells. \u00a0This result was obtained in 3 consecutive experiments. \u00a0The astroglial membrane properties recorded from the translocating RG cell, and its daughters, stands in contrast to the neuronal membrane properties I readily and consistently recorded from daughter cells produced by IP cell divisions.<\/h6>\n<h4 style=\"text-align: center;\">Vertical divisions produce translocating radial glial cells.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-7\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-tRG1.mp4?_=7\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-tRG1.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec2-tRG1.mp4<\/a><\/video><\/div>\n<h6 style=\"text-align: justify;\">This time-lapse movie shows another vertical RG cell division yielding morphologically distinct daughter cells. The division at t=4h was vertical, and yielded one daughter cell that retained the pial fiber (red arrowhead), and a second daughter cell (red arrow) that remained close to the mother cell over 100+ hours of time-lapse recording. The RG cell loses contact with the ventricle during recording. The ventricular contacting process appears to degrade at the t=39h and t=45h time points, and was no longer\u00a0visible after that point.<\/h6>\n<h4 style=\"text-align: center;\">E13 Rat RG cell division.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-8\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-e13-RG.mp4?_=8\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-e13-RG.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-e13-RG.mp4<\/a><\/video><\/div>\n<h6 style=\"text-align: justify;\">This time-lapse movie was recorded from a radial glial cell in the E13 rat, during early stages of cortical development. \u00a0Cytokinesis begins at the t=30m timepoint, and this RG cell division produces two daughter cells that have similar morphology.<\/h6>\n<h4 style=\"text-align: center;\">Symmetric IP Cell Division in the SVZ.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-9\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/supplamental-movie1.mp4?_=9\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/supplamental-movie1.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/supplamental-movie1.mp4<\/a><\/video><\/div>\n<h6>In contrast to the RG cell divisions at the ventricle that were mostly vertical &#8211; 34\/36 time-lapse recordings of RG cells were vertical &#8211; I found that IP cells were more likely to divide with a horizontal orientation during neurogenic stages of cortical development. The horizontal division of IP cells produced symmetrically fated daughter cells, as classified by morphology, behavior and physiological recordings.<\/h6>\n<h4 style=\"text-align: center;\">Pial fiber varicosities enter RG cells before division.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-10\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-migrating-vess.mp4?_=10\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-migrating-vess.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-migrating-vess.mp4<\/a><\/video><\/div>\n<h6>In the time-lapse recordings I observed that the RG cell pial fiber became very thin during M-phase of division. The fiber became so thin that in some cases I had to either expose the cell to higher laser power, or scan for a longer time to visualize the fiber. Of interesting, I noticed that as the fiber became thinner I could detect multiple varicosities traveling along the fiber in both directions but most often towards the RG cell soma. In many cases one or more of the varicosities entered the RG cell soma prior to division.<\/h6>\n<h1>More Videos<\/h1>\n<h4 style=\"text-align: center;\">Radial glial cell in G2-phase.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-11\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-dividing-rg.mp4?_=11\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-dividing-rg.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-dividing-rg.mp4<\/a><\/video><\/div>\n<h6>This time-lapse movie shows a radial glial cell entering G2-phase of the cell cycle. During G2 radial glial cells that maintain contact with the surface of the ventricle approach the ventricle where they will undergo cytokinesis.<\/h6>\n<h4 style=\"text-align: center;\">How to Make a Movie.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-12\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-how-to.mp4?_=12\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-how-to.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/11\/rec-how-to.mp4<\/a><\/video><\/div>\n<h6>Making a time-lapse movie is not actually as technically challenging as you might think.<\/h6>\n<h4 style=\"text-align: center;\">Sunrise over Sunflowers.<\/h4>\n<div style=\"width: 625px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-47-13\" width=\"625\" height=\"352\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/Sunflower-Sunrise.mp4?_=13\" \/><a href=\"http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/Sunflower-Sunrise.mp4\">http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/Sunflower-Sunrise.mp4<\/a><\/video><\/div>\n<h6>June 21, 2015 in Davis, CA<\/h6>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>2004 Nature Neuroscience Publication [masterslider id=&#8221;8&#8243;] 2008 Journal of Comparative Neurology Publication [masterslider id=&#8221;10&#8243;] \u00a0More Videos [masterslider id=&#8221;11&#8243;] 2004 Nature Neuroscience Publication Translocation of a radial glial cell out of the ventricular zone. http:\/\/ventricular.org\/StephenNoctor\/wp-content\/uploads\/2015\/10\/tRG-the-first-Again.mp4 Among other projects I worked on as a graduate student in Sharon Juliano&#8217;s lab, I studied radial glial (RG) cell morphology [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/full-width.php","meta":{"footnotes":""},"class_list":["post-47","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/pages\/47","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/comments?post=47"}],"version-history":[{"count":68,"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/pages\/47\/revisions"}],"predecessor-version":[{"id":800,"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/pages\/47\/revisions\/800"}],"wp:attachment":[{"href":"https:\/\/ventricular.org\/StephenNoctor\/wp-json\/wp\/v2\/media?parent=47"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}